Khoebo ea sterility ea lijo tsa makotikoting e bua ka boemo bo batlang bo nyopa moo ho se nang likokoana-hloko le likokoana-hloko tse se nang pathogenic tse ka ikatisang ka lijo tse entsoeng ka makotikoting ka mor'a hore lijo tse entsoeng ka makotikoting li fumane phekolo e itekanetseng ea mocheso, ke ntho ea bohlokoa e hlokahalang bakeng sa lijo tse entsoeng ka makotikoting. bophelo bo bolelele ba shelofo motheong oa ho netefatsa polokeho ea lijo le boleng.Thekiso ea khoebo ea lijo tse entsoeng ka makotikoting litekong tsa likokoana-hloko tsa lijo li khetholloa ke ho sterility e lekanyelitsoeng, ha ho na likokoana-hloko tsa pathogenic, 'me ha ho likokoana-hloko tse ka ikatisang ka makotikoti mocheso oa kamore.
Bakeng sa ho fihlela maemo a amohelehang a khoebo, mokhoa oa tlhahiso ea lijo tse ka makotikoting hangata o kenyelletsa lits'ebetso tse joalo ka phetisetso ea lihlahisoa tse tala, ho kenya makotikoti, ho tiisa, ho thibela likokoana-hloko hantle le ho paka.Baetsi ba nang le theknoloji e tsoetseng pele ea tlhahiso le litlhoko tsa taolo ea boleng bo phahameng ba na le mekhoa e rarahaneng le e phethahetseng ea tlhahiso.
Theknoloji ea tlhahlobo ea sterility e entsoeng ka makotikoting ea tlhahlobo ea likokoana-hloko ea lijo e se e felile, 'me tlhahlobo ea ts'ebetso ea eona e ikhethileng e thusa ho sebelisa theknoloji ena hamolemo ts'ebetsong e sebetsang ho netefatsa polokeho ea lijo tsa makotikoting.Ts'ebetso e ikhethileng ea tlhahlobo ea sterility ea makotikoting ea tlhahlobo ea likokoana-hloko tsa lijo ke e latelang (likarolo tse ling tse thata tsa tlhahlobo ea motho oa boraro li ka ba le lintho tse ngata tsa tlhahlobo):
1. Tloaelo ea baktheria ea makotikoting
Tloaelo ea baktheria ea makotikoting ke e 'ngoe ea mekhoa ea bohlokoa ea tlhahlobo ea khoebo ea sterility ea lijo tse entsoeng ka makotikoting.Ka ho hlaolela litaba tsa lisampole tse ka makotikoting ka botsebi, le ho hlahlobisisa le ho lekola likolone tsa baktheria tse holileng, likarolo tsa likokoana-hloko lijong tse ka makotikoting li ka hlahlojoa.
Likokoana-hloko tse tloaelehileng tsa pathogenic ka makotikoti li kenyelletsa empa ha li felle feela ho libaktheria tsa thermophilic, tse kang Bacillus stearothermophilus, Bacillus coagulans, Clostridium saccharolyticus, Clostridium niger, joalo-joalo;libaktheria tsa mesophilic anaerobic, tse kang chefo ea botulinum Clostridium, Clostridium spoilage, Clostridium butyricum, Clostridium pasteurianum, joalo-joalo;Libaktheria tsa Mesophilic aerobic, tse kang Bacillus subtilis, Bacillus cereus, joalo-joalo;Libaktheria tse sa hlahiseng spore tse kang Escherichia coli, Streptococcus, yeast Le hlobo, hlobo e thibelang mocheso joalo-joalo.Pele o etsa moetlo oa baktheria oa makotikoting, etsa bonnete ba hore o lekanya pH ea lekotikoti e le hore u khethe mokhoa o nepahetseng.
2. Sampling ea thepa ea teko
Mokhoa oa ho etsa lisampole hangata o sebelisoa ho etsa sampole ea lisebelisoa tsa liteko tsa lijo tse ka makotikoting.Ha ho etsoa liteko tse ngata tsa lijo tse entsoeng ka makotikoting, sampole hangata e etsoa ho latela lintlha tse kang moetsi, letšoao la khoebo, mefuta e fapaneng, mohloli oa lijo tse entsoeng ka makotikoting kapa nako ea tlhahiso.Bakeng sa makotikoti a sa tloaelehang joalo ka makotikoti a mafome, makotikoti a silafalitsoeng, makotikoti le ho ruruha ho potolohang ha barekisi le matlong a polokelo, lisampole tse khethehileng hangata li etsoa ho latela maemo.Ke tlhokahalo ea mantlha bakeng sa sampole ea lisebelisoa tsa liteko ho khetha mokhoa o nepahetseng oa sampole ho latela maemo a nnete, e le ho fumana lisebelisoa tsa liteko tse bonts'ang boleng ba lijo tse ka makotikoting.
3. Beha mohlala
Pele ho boloka sampole, ho hlokahala tšebetso e kang ho bekha, ho futhumala le ho bula makotikoti.Beha boima ba letlooa ka thoko, ho latela mofuta oa can, e hloka ho nepahala ho isa ho 1g kapa 2g.Li kopantsoe le pH le mocheso, makotikoti a bolokiloe mocheso o sa khaotseng oa matsatsi a 10;makotikoti a mafura kapa a lutlang nakong ea ts'ebetso a lokela ho khethoa hang-hang bakeng sa tlhahlobo.Ka mor'a hore mokhoa oa ho boloka mocheso o felile, beha lekotikoti mochesong oa kamore bakeng sa ho bula aseptic.Ka mor'a ho bula lekotikoti, sebelisa lisebelisoa tse loketseng ho nka 10-20 mg ea litaba esale pele sebakeng se nyopa, ho e fetisetsa ka sejaneng se hloekisitsoeng, ebe u se boloka ka sehatsetsing.
4.Setso sa lijo tse nang le asiti e tlase
Ho lengoa ha lijo tse nang le asiti e tlase ho hloka mekhoa e ikhethileng: ho lema moro o pherese oa brompotassium ka 36 ° C, ho lengoa moro oa pherese oa brompotassium ka 55 ° C, le ho lema nama e phehiloeng ka mocheso oa 36 ° C.Liphetho lia tlotsoa le ho silafatsoa, 'me tlhahlobo e nepahetseng haholoanyane e hlophisoa ka mor'a tlhahlobo ea microscopic, e le ho netefatsa bonnete ba sepheo sa teko ea ho khetholla mefuta ea baktheria lijong tse nang le asiti e tlase.Ha u lema sebakeng se bohareng, tsepamisa maikutlo ho shebelleng tlhahiso ea asiti le khase ea li-colone tsa likokoana-hloko tse bohareng, hammoho le ponahalo le 'mala oa likolone, e le ho netefatsa mefuta e itseng ea likokoana-hloko lijong.
5. Tlhahlobo ea microscopic
Tlhahlobo ea microscopic smear ke mokhoa o sebelisoang ka ho fetesisa oa tlhahlobo bakeng sa tlhahlobo ea sterility ea khoebo ea makotikoting, e hlokang hore bahlahlobi ba nang le boiphihlelo ba boleng ba phethe.Sebakeng se sa hloekang, u sebelisa ts'ebetso ea aseptic, tlotsa mokelikeli oa baktheria oa likokoana-hloko tse ka har'a lisampole tse entsoeng ka makotikoting tse hlahisitsoeng ka mocheso o sa feleng bohareng, 'me u shebe ponahalo ea libaktheria tlas'a microscope e matla haholo, fumana mefuta ea likokoana-hloko ka mokelikeli oa baktheria.Ho hlahloba, le ho hlophisa mohato o latelang oa setso se hloekisitsoeng le boitsebahatso ho netefatsa ka ho eketsehileng mofuta oa libaktheria tse ka har'a lekotikoti.Mohato ona o hloka boleng bo phahameng ka ho fetesisa ba bahlahlobi, hape e fetohile sehokelo se ka lekang ka ho fetisisa tsebo le litsebo tsa litsebi tsa bahlahlobi.
6. Teko ea temo ea lijo tse nang le asiti e nang le pH e ka tlase ho 4.6
Bakeng sa lijo tse nang le asiti tse nang le pH e tlase ho 4.6, tlhahlobo ea libaktheria tse chefo hangata ha e sa hlokahala.Ts'ebetsong e khethehileng ea ho lema, ntle le ho sebelisa moro o nang le asiti joalo ka seaplane, ho boetse hoa hlokahala ho sebelisa moro o tsoang ho 'mela e le mokhoa oa ho lema.Ka ho tlotsa le ho hlahloba microscopic ea likolone tsa baktheria tse holileng, mefuta ea libaktheria tse ka har'a makotikoti a acid e ka khethoa, e le ho tsoela pele ho etsa tlhahlobo e nepahetseng le ea 'nete ea polokeho ea lijo tsa makotikoti a acid.
Nako ea poso: Aug-10-2022